Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: Surface markers and cytokines expressed by naïve and memory T-cells

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Expressing

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: .Stimulated antigen-experienced T-cells gain effector function without cell division. PBMC from healthy adult donors depleted of CD45RA+ T-cells, were stained with 0·25 µm CFSE and were then stimulated for 4 days with PHA or SEB, or for 6 days by MLR. Shown is a dot plot panel of surface markers and cytokine production for antigen-experienced CD4+ or CD8+ gated T-cells.

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: IL-7 drives proliferation of naïve T-cells in MHC independent fashion. Cord blood PBMC were stained with 0·25 µm CFSE and were then cultured for 9 days in 10 ng/ml IL-7 with isotypic control antibodies (a) or 10 µg/ml blocking antibodies recognizing HLA A, B, C, DR, DP, and DQ (b) Dot plots of CD4+ or CD8+ gated T-cells show expression patterns of CD45RA, and CD45RO with respect to cell division. Boxed areas of CD4 and CD8 plots represent the percentage of cultured cells that had proliferated more than three times for IL-7 alone (a) and IL-7+ anti-MHC antibodies (b).

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining, Cell Culture, Control, Blocking Assay, Expressing

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: IL-2 is Required for IL-7 induced expansion of memory T-Cells present in umbilical cord blood. Cord blood PBMC were stained with 0·25 µm CFSE and were then cultured for 9 days in 10 ng/ml IL-7 with or without 10 µg/ml depleting antibodies to IL-2. After culturing, PBMC were stained with CD4 and CD45RO or CD45RA, and CD8 and CD45RA or CD45RO. Dot plots of CD4+ or CD8+ T-cells show expression patterns of CD45RA, CD45RO, CD25, and IFN-γ with respect to cell division.

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining, Cell Culture, Expressing